Inhibitory effects on HepG2 cell proliferation and induction of cell cycle arrest by Chromolaena odorata leaf extract and fractions

Concern about the side effects of liver cancer treatment has driven studies on anticancer to find compounds from plants that can act as chemotherapy. The activity Chromolaena odorata against colorectal cancer, lung leukemia, cervical breast and been proven. However, this plant’s mechanism inhibit cell growth is still undetermined. This study aims investigate C. HepG2 cells. Extraction leaves was done by maceration method using 80% ethanol further fractionated. Total flavonoid major compound crude extract were determined aluminum chloride colorimetric assay Liquid Chromatography-Mass Spectrometry method. IC 50 proliferation analysis performed MTT assay. Cell cycle analyzed flowcytometry. 1.95% such 5,7,8,3ʹ,4ʹ-Pentamethoxyflavonone, 1-Carboethoxy-β-carboline, 3-Methylcanthin-2, 6- dion, Canthin-6-one found in . significantly lower after 72 hours incubation with ½ fractions. cells treated fractions accumulated G0-G1 phase. These results indicated could induce arrest.